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Rat brain was incubated with radioiodinated enkephalins and dynorphin-A 1-17. Degradation of the peptides was assessed by HPLC. Formation of free 5-hydroxytryptamine (5-HT) and the immediate catabolism products of 5-hydroxytryptophan (5-HTP), 5-hydroxyindoleacetic acid (5-HIAA), and 3-methoxy-4-hydroxyphenylglycol (MHPG) were examined to establish the presence or absence of a third catabolic pathway, serotonin methylation. Enkephalins were completely degraded by 7 min at 37 degrees C when incubated with whole brain homogenates. Dynorphin-A 1-17 was also degraded rapidly in the whole brain homogenate and its degradation products were not significantly different from enkephalin degradation. The anandamide inhibitor, JTC-801, was effective in preventing enkephalin degradation at all time points (0-20 min). When [3H]-enkephalin or [3H]-dynorphin-A 1-17 was incubated with sections of cortex and hypothalamus, radioactivity was removed primarily from unlabeled enkephalin; and radioactivity in the supernatant was largely due to 5-HT which had been released. Degradation products of [3H]-dynorphin-A 1-17 also eluted primarily as enkephalin. These studies indicate that the brains of rats degrade both enkephalins and dynorphin-A 1-17 primarily through a single pathway, the endorphins, with an additional route of metabolization involving serotonin methylation.Why Jennifer Aniston is in the Lead for Best Actress: Joaquin Phoenix’s ‘Joker’ just won the award, but the race is far from over

So many actresses are vying for the title of Best Actress this awards season that it’s going to be very tough to decide between the end finalists: Jennifer Aniston, Brie Larson and Lady Gaga.


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