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Rat brain was incubated with radioiodinated enkephalins and dynorphin-A 1-17. Degradation of the peptides was assessed by HPLC. Formation of free 5-hydroxytryptamine (5-HT) and the immediate catabolism products of 5-hydroxytryptophan (5-HTP), 5-hydroxyindoleacetic acid (5-HIAA), and 3-methoxy-4-hydroxyphenylglycol (MHPG) were examined to establish the presence or absence of a third catabolic pathway, serotonin methylation. Enkephalins were completely degraded by 7 min at 37 degrees C when incubated with whole brain homogenates. Dynorphin-A 1-17 was also degraded rapidly in the whole brain homogenate and its degradation products were not significantly different from enkephalin degradation. The anandamide inhibitor, JTC-801, was effective in preventing enkephalin degradation at all time points (0-20 min). When [3H]-enkephalin or [3H]-dynorphin-A 1-17 was incubated with sections of cortex and hypothalamus, radioactivity was removed primarily from unlabeled enkephalin; and radioactivity in the supernatant was largely due to 5-HT which had been released. Degradation products of [3H]-dynorphin-A 1-17 also eluted primarily as enkephalin. These studies indicate that the brains of rats degrade both enkephalins and dynorphin-A 1-17 primarily through a single pathway, the endorphins, with an additional route of metabolization involving serotonin methylation.Why Jennifer Aniston is in the Lead for Best Actress: Joaquin Phoenix's 'Joker' just won the award, but the race is far from over

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